Molecular Nutrition and Food Research [IF:5.309]
Lycium ruthenicum anthocyanins attenuate high‐fat diet‐induced colonic barrier dysfunction and inflammation in mice by modulating the gut microbiota
Scope: Gut barrier dysfunction and inflammation originating from a dysbiotic gut microbiota (GM) are strongly associated with a high‐fat diet (HFD). Anthocyanins from Lycium ruthenicum (ACs) have shown antiobesity effects through modulating the GM. However, the mechanism linking the antiobesity effects of ACs and GM modulation remains obscure.
Methods and results: To investigate the ameliorative effects of ACs on colonic barrier dysfunction and inflammation, mice were fed an HFD with or without ACs at doses of 50, 100 and 200 mg/kg for 12 weeks. AC supplementation reduced weight gain, enriched short‐chain fatty acid (SCFA)‐producing bacteria (e.g., Ruminococcaceae, Muribaculaceae, Akkermansia, Ruminococcaceae_UCG‐014, and Bacteroides) and SCFA content, depleted endotoxin‐producing bacteria (e.g., Helicobacter and Desulfovibrionaceae), and decreased endotoxin (i.e., lipopolysaccharides) levels. SCFAs substantially activated G protein‐coupled receptors (GPRs), inhibited histone deacetylases (HDAC), increased intestinal tight junction mRNA and protein expression levels, reduced intestinal permeability, and protected intestinal barrier integrity in HFD‐induced mice. These effects mitigated intestinal inflammation by inhibiting the LPS/NF‐κB/TLR4 pathway.
Conclusion: These data indicated that ACs can mitigate colonic barrier dysfunction and inflammation, induce the SCFA production and inhibit endotoxin production by modulating the GM in HFD‐fed mice. This finding provides a clue for understanding the antiobesity effects of ACs.